Hotspot

petA-psbJ

LSC · 68068-69182 · 1115 bp

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Pi 0.269
MarkerSeek score 75.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1115
variable_sites
189
pi
0.269
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.63
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TACAGCGGCGGGTATAGTAA AACAGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 1648-2050 bp 1.0 91.8
petA-psbJ_p2 TACAGCGGCGGGTATAGTAA ACAGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1647-2049 bp 1.0 91.8
petA-psbJ_p3 TACAGCGGCGGGTATAGTAA ACGATGTTTTTGGAAGCCCT 50.0 45.0 58.0 58.0 1715-2117 bp 1.0 91.8
petA-psbJ_p4 TACAGCGGCGGGTATAGTAA ATGTTTTTGGAAGCCCTCGT 50.0 45.0 58.0 58.0 1712-2114 bp 1.0 91.8
petA-psbJ_p5 TACAGCGGCGGGTATAGTAA CTGGCTGTACCTACCGTTTC 50.0 55.0 58.0 58.0 1527-1929 bp 1.0 91.8