Hotspot

ndhK-ndhC

LSC · 55053-55059 · 7 bp

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Pi 0.143

MarkerSeek score 75.90

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 7
variable_sites: 1
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AAACTGGAGAGTCTTGACCA	GCTCTTGAATATTCATCAGACA	45.0	36.4	56.0	54.0	157 bp	1.0	40.5
ndhK-ndhC_p2	AACTGGAGAGTCTTGACCAA	GCTCTTGAATATTCATCAGACA	45.0	36.4	56.0	54.0	156 bp	1.0	40.5
ndhK-ndhC_p3	AAACTGGAGAGTCTTGACCA	GCTCTTGAATATTCATCAGACAA	45.0	34.8	56.0	54.8	157 bp	1.0	40.5
ndhK-ndhC_p4	AACTGGAGAGTCTTGACCAA	GCTCTTGAATATTCATCAGACAA	45.0	34.8	56.0	54.8	156 bp	1.0	40.5
ndhK-ndhC_p5	AAACTGGAGAGTCTTGACCAA	GCTCTTGAATATTCATCAGACA	42.9	36.4	56.8	54.0	157 bp	1.0	40.5