Hotspot

rps4-trnT-UGU

LSC · 50460-50938 · 479 bp

Back to Ventilago

Pi 0.0194
MarkerSeek score 69.60
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
479
variable_sites
9
pi
0.0194
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 ATCACTTCCGGCTCTAGGTT GGTTCGATTCCGATAGCTGG 50.0 55.0 58.1 58.2 594-604 bp 1.0 80.4
rps4-trnT-UGU_p2 ATCACTTCCGGCTCTAGGTT ATGGTCATCGGTTCGATTCC 50.0 50.0 58.1 57.8 603-613 bp 1.0 80.2
rps4-trnT-UGU_p3 GAAACGAGGCCCTCTGTAAC GGTTCGATTCCGATAGCTGG 55.0 55.0 58.3 58.2 528-538 bp 1.0 79.7
rps4-trnT-UGU_p4 ATCACTTCCGGCTCTAGGTT GGGGTTAGAGCATCGCATTT 50.0 50.0 58.1 58.3 631-641 bp 1.0 79.4
rps4-trnT-UGU_p5 AGATCACTTCCGGCTCTAGG GGTTCGATTCCGATAGCTGG 55.0 55.0 58.3 58.2 596-606 bp 1.0 79.1