Hotspot

petN-psbM

LSC · 31919-32779 · 861 bp

Back to Ventilago

Pi 0.00826

MarkerSeek score 65.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 861
variable_sites: 7
pi: 0.00826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCGTTCT	50.0	50.0	58.2	58.2	1022-1474 bp	1.0	78.4
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCGTTCT	50.0	50.0	57.6	58.2	961-1413 bp	1.0	76.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCGTTC	50.0	50.0	58.2	58.5	1023-1475 bp	1.0	76.1
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCGTTC	50.0	50.0	57.6	58.5	962-1414 bp	1.0	74.6
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	GCTACTGCACTGTTCGTTCT	50.0	50.0	57.1	58.2	1023-1475 bp	1.0	72.5