Hotspot

rps16-trnQ-UUG

LSC · 6676-7665 · 990 bp

Back to Ventilago

Pi 0.0408
MarkerSeek score 66.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
990
variable_sites
25
pi
0.0408
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.62
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG TGTCCAATAGAAATGGGGCG 50.0 50.0 57.9 57.9 865-1141 bp 1.0 84.9
rps16-trnQ-UUG_p2 AACGGATCGTGTCCTTCAAG GTCCAATAGAAATGGGGCGT 50.0 50.0 57.9 57.9 864-1140 bp 1.0 84.9
rps16-trnQ-UUG_p3 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 804-1080 bp 1.0 84.9
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TGTCCAATAGAAATGGGGCG 45.0 50.0 58.1 57.9 848-1124 bp 1.0 84.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTCCAATAGAAATGGGGCGT 45.0 50.0 58.1 57.9 847-1123 bp 1.0 84.5