Hotspot

rpoC2

LSC · 16902-21017 · 4116 bp

Back to Veitchia

Pi 0.000324

MarkerSeek score 41.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4116
variable_sites: 2
pi: 0.000324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ATCGTGATGGAGAAAGCACC	GATTTGCTGGGTCCCTTAGG	50.0	55.0	58.0	57.9	243 bp	1.0	80.0
rpoC2_p2	ATCGTGATGGAGAAAGCACC	GGATTTGCTGGGTCCCTTAG	50.0	55.0	58.0	57.9	244 bp	1.0	79.9
rpoC2_p3	CGTGATGGAGAAAGCACCAA	GATTTGCTGGGTCCCTTAGG	50.0	55.0	58.5	57.9	241 bp	1.0	76.4
rpoC2_p4	CGTGATGGAGAAAGCACCAA	GGATTTGCTGGGTCCCTTAG	50.0	55.0	58.5	57.9	242 bp	1.0	76.4
rpoC2_p5	ATCGTGATGGAGAAAGCACC	ATTAGGGATTTGCTGGGTCC	50.0	50.0	58.0	57.2	249 bp	1.0	74.4