Hotspot

rpl32-trnL-UAG

SSC · 115335-116098 · 764 bp

Back to Veitchia

Pi 0.00175

MarkerSeek score 49.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 764
variable_sites: 2
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTTTCCACCGGACATTCAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.4	58.0	857-885 bp	1.0	78.4
rpl32-trnL-UAG_p2	GGTTTCCACCGGACATTCAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.4	58.0	858-886 bp	1.0	78.4
rpl32-trnL-UAG_p3	GGTTTCCACCGGACATTCAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.4	57.9	867-895 bp	1.0	77.4
rpl32-trnL-UAG_p4	GGTTTCCACCGGACATTCAA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.4	57.8	866-894 bp	1.0	76.9
rpl32-trnL-UAG_p5	GGTTTCCACCGGACATTCAA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.4	57.8	865-893 bp	1.0	76.9