Hotspot

clpP-psbB

LSC · 73513-73961 · 449 bp

Back to Veitchia

Pi 0.0104

MarkerSeek score 49.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 7
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	627 bp	1.0	80.0
clpP-psbB_p2	CGCACTATACGTCAACCCAA	TATGAACGCGATACCAAGGC	50.0	50.0	58.0	58.1	553 bp	1.0	79.9
clpP-psbB_p3	CGCACTATACGTCAACCCAA	TGGACAGAAAGCAATCGACC	50.0	50.0	58.0	58.2	593 bp	1.0	79.4
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	545 bp	1.0	79.1
clpP-psbB_p5	CGCACTATACGTCAACCCAA	CAATCGACCGGGATCATTCA	50.0	50.0	58.0	57.8	582 bp	1.0	79.0