Hotspot

rpl20-rps12

LSC · 70469-71179 · 711 bp

Back to Veitchia

Pi 0.000938

MarkerSeek score 47.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 711
variable_sites: 1
pi: 0.000938
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGACGTTTCCGAGCTGTATA	TCTCCCGCTCTTAGAGGATG	50.0	55.0	57.5	58.0	805-806 bp	1.0	76.4
rpl20-rps12_p2	CGACGTTTCCGAGCTGTATA	GAGGAACATGCACTAGGGTG	50.0	55.0	57.5	58.0	774-775 bp	1.0	76.3
rpl20-rps12_p3	CGACGTTTCCGAGCTGTATA	GTCGAGGAACATGCACTAGG	50.0	55.0	57.5	58.1	777-778 bp	1.0	75.9
rpl20-rps12_p4	TCCGAGCTGTATATCCTCGT	TCTCCCGCTCTTAGAGGATG	50.0	55.0	57.4	58.0	798-799 bp	1.0	75.6
rpl20-rps12_p5	TCCGAGCTGTATATCCTCGT	GAGGAACATGCACTAGGGTG	50.0	55.0	57.4	58.0	767-768 bp	1.0	75.5