Hotspot

psbZ-trnG-UCC

LSC · 36296-36583 · 288 bp

Back to Veitchia

Pi 0.00231

MarkerSeek score 40.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 1
pi: 0.00231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TGCTTCTTCTGATGGTTGGT	TACTTCATCGTGGGCGGATA	45.0	50.0	57.0	58.3	480 bp	1.0	70.1
psbZ-trnG-UCC_p2	TGCTTCTTCTGATGGTTGGT	ACATTACTTCATCGTGGGCG	45.0	50.0	57.0	58.3	484 bp	1.0	69.7
psbZ-trnG-UCC_p3	TGCTTCTTCTGATGGTTGGT	GATCGAACCCGCATCTTCTC	45.0	55.0	57.0	58.5	455 bp	1.0	68.5
psbZ-trnG-UCC_p4	GCTTCTTCTGATGGTTGGTCA	TACTTCATCGTGGGCGGATA	47.6	50.0	58.2	58.3	479 bp	1.0	68.0
psbZ-trnG-UCC_p5	TGCTTCTTCTGATGGTTGGTC	TACTTCATCGTGGGCGGATA	47.6	50.0	58.2	58.3	480 bp	1.0	68.0