Hotspot

trnT-GGU-psbD

LSC · 32068-33114 · 1047 bp

Back to Veitchia

Pi 0.00127

MarkerSeek score 47.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1047
variable_sites: 2
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1211-1216 bp	1.0	77.7
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1206-1211 bp	1.0	77.7
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	50.0	50.0	58.1	58.4	1224-1229 bp	1.0	76.6
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	GCCAGACCAGCCTACAAAAA	50.0	50.0	57.9	58.4	1219-1224 bp	1.0	76.6
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1210-1215 bp	1.0	76.6