Hotspot

trnD-GUC-trnY-GUA

LSC · 31121-31504 · 384 bp

Back to Veitchia

Pi 0.00868

MarkerSeek score 39.90

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 5
pi: 0.00868
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	55.0	50.0	57.9	58.0	563 bp	1.0	79.8
trnD-GUC-trnY-GUA_p2	GTGCTCTGACCGATTGAACT	AATTCTTCCTGGGTCGATGC	50.0	50.0	57.9	58.0	506 bp	1.0	79.7
trnD-GUC-trnY-GUA_p3	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	55.0	55.0	57.9	58.4	530 bp	1.0	77.4
trnD-GUC-trnY-GUA_p4	ATCGGATCCTAGTTCGGGAC	AATTCTTCCTGGGTCGATGC	55.0	50.0	58.4	58.0	566 bp	1.0	77.3
trnD-GUC-trnY-GUA_p5	GTGCTCTGACCGATTGAACT	GGGGACGGACTGTAAATTCG	50.0	55.0	57.9	58.4	473 bp	1.0	77.3