Hotspot

trnC-GCA-petN

LSC · 28494-29099 · 606 bp

Back to Veitchia

Pi 0.0011

MarkerSeek score 47.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TAAAGCAGCCCAAGTGAGAC	50.0	50.0	57.5	57.8	686 bp	1.0	74.4
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	ACCATTAAAGCAGCCCAAGT	50.0	45.0	57.5	57.7	691 bp	1.0	73.4
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.5	57.6	741 bp	1.0	73.1
trnC-GCA-petN_p4	CCAGTTCAAATCCGGGTGTC	TAAAGCAGCCCAAGTGAGAC	55.0	50.0	58.8	57.8	663 bp	1.0	72.0
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	GCAGCCCAAGTGAGACTTAC	50.0	55.0	57.5	58.6	682 bp	1.0	71.5