Hotspot

trnK-UUU-rps16

LSC · 4112-4832 · 721 bp

Back to Veitchia

Pi 0.00185
MarkerSeek score 47.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
721
variable_sites
2
pi
0.00185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCTGGACGAATCTGTTGC GCTCAACCTACGGGAACTG 50.0 57.9 57.9 57.9 892 bp 1.0 70.4
trnK-UUU-rps16_p2 GTCTGGACGAATCTGTTGCT GCTCAACCTACGGGAACTG 50.0 57.9 57.9 57.9 891 bp 1.0 70.4
trnK-UUU-rps16_p3 AGTCTGGACGAATCTGTTGC GTGCTCAACCTACGGGAAC 50.0 57.9 57.9 58.2 894 bp 1.0 70.2
trnK-UUU-rps16_p4 GTCTGGACGAATCTGTTGCT GTGCTCAACCTACGGGAAC 50.0 57.9 57.9 58.2 893 bp 1.0 70.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GCTCAACCTACGGGAACTG 50.0 57.9 58.2 57.9 835 bp 1.0 69.7