Hotspot

rps3-rpl22

LSC · 87001-87129 · 129 bp

Back to Vauquelinia

Pi 0.0245

MarkerSeek score 59.00

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 5
pi: 0.0245
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TTGCACCAAGTCTGAAACCA	TCCTATAAAAAGATCCACTTGTCA	45.0	33.3	57.9	55.5	80-312 bp	1.0	41.7
rps3-rpl22_p2	GTTGCACCAAGTCTGAAACC	TCCTATAAAAAGATCCACTTGTCA	50.0	33.3	57.5	55.5	80-313 bp	1.0	41.7
rps3-rpl22_p3	AATGATGACCTTGGGTTGCA	TCCTATAAAAAGATCCACTTGTCA	45.0	33.3	57.7	55.5	80-327 bp	1.0	41.6
rps3-rpl22_p4	ATGATGACCTTGGGTTGCAC	TCCTATAAAAAGATCCACTTGTCA	50.0	33.3	58.5	55.5	80-326 bp	1.0	41.6
rps3-rpl22_p5	TGCACCAAGTCTGAAACCAA	TCCTATAAAAAGATCCACTTGTCA	45.0	33.3	57.9	55.5	192-311 bp	1.0	41.5