Hotspot

psaJ-rpl33

LSC · 70603-71084 · 482 bp

Back to Vauquelinia

Pi 0.0131

MarkerSeek score 59.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 482
variable_sites: 9
pi: 0.0131
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	189-586 bp	1.0	75.4
psaJ-rpl33_p2	TCCGGATGCGTTGATATTCC	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	188-613 bp	1.0	75.2
psaJ-rpl33_p3	TCCGGATGCGTTGATATTCC	CGGACATCTTTACCCTTAGCC	50.0	52.4	57.8	58.2	189-542 bp	1.0	75.0
psaJ-rpl33_p4	TCCGGATGCGTTGATATTCC	ACGATTTCGAACACAACTGG	50.0	45.0	57.8	56.7	188-585 bp	1.0	73.6
psaJ-rpl33_p5	TCCGGATGCGTTGATATTCC	CGATTTCGAACACAACTGGT	50.0	45.0	57.8	56.7	187-584 bp	1.0	73.6