Hotspot

ndhC-trnV-UAC

LSC · 53997-54635 · 639 bp

Back to Vauquelinia

Pi 0.00782

MarkerSeek score 54.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 8
pi: 0.00782
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCCGAAAT	GCACGTTGGGTCTTTGAAAC	50.0	50.0	57.9	58.2	188-834 bp	1.0	82.5
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCCGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	187-770 bp	1.0	82.4
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCCGAAAT	AATGCACGTTGGGTCTTTGA	50.0	45.0	57.9	58.3	80-837 bp	1.0	81.4
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCCGAAAT	ATGCACGTTGGGTCTTTGAA	50.0	45.0	57.9	58.3	80-836 bp	1.0	81.4
ndhC-trnV-UAC_p5	CTGGTCCTTTGCTAATCGGG	GCACGTTGGGTCTTTGAAAC	55.0	50.0	58.3	58.2	189-851 bp	1.0	81.0