Hotspot

psbZ-trnG-GCC

LSC · 38759-39269 · 511 bp

Back to Vauquelinia

Pi 0.00844

MarkerSeek score 59.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 7
pi: 0.00844
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	334-703 bp	1.0	81.4
psbZ-trnG-GCC_p2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	320-689 bp	1.0	81.2
psbZ-trnG-GCC_p3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.0	57.8	290-659 bp	1.0	75.6
psbZ-trnG-GCC_p4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.2	57.8	321-690 bp	1.0	74.4
psbZ-trnG-GCC_p5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.2	57.8	335-704 bp	1.0	74.0