Hotspot

trnR-UCU-atpA

LSC · 9933-10554 · 622 bp

Back to Vauquelinia

Pi 0.00773

MarkerSeek score 54.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 8
pi: 0.00773
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	ACAATCTCAATCATCCCCGC	50.0	50.0	58.0	58.0	1193-1240 bp	1.0	82.8
trnR-UCU-atpA_p2	CCTAGCCTTCCAAGCTAACG	ACAATCTCAATCATCCCCGC	55.0	50.0	58.1	58.0	1196-1243 bp	1.0	82.6
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	TTCGGTTTCCAGGGTAGGAT	50.0	50.0	58.0	58.0	1373-1420 bp	1.0	82.3
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	TCAGACCGGCCATTAATGTG	50.0	50.0	58.0	58.0	1398-1445 bp	1.0	82.2
trnR-UCU-atpA_p5	CCTAGCCTTCCAAGCTAACG	TCAGACCGGCCATTAATGTG	55.0	50.0	58.1	58.0	1401-1448 bp	1.0	82.0