Hotspot

matK-trnK-UUU

LSC · 3529-4240 · 712 bp

Back to Vauquelinia

Pi 0.00328

MarkerSeek score 58.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 4
pi: 0.00328
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.2	58.2	836-843 bp	1.0	73.6
matK-trnK-UUU_p2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	835-842 bp	1.0	73.6
matK-trnK-UUU_p3	CCATGATCATGAGCAAGTGC	TGGTTTGGATTGCATTGTGC	50.0	45.0	57.2	57.8	929-936 bp	1.0	73.5
matK-trnK-UUU_p4	CCATGATCATGAGCAAGTGC	TTGGGTTGCTAACTCAACGG	50.0	50.0	57.2	58.4	851-858 bp	1.0	71.7
matK-trnK-UUU_p5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.2	57.5	849-856 bp	1.0	71.2