Hotspot

trnH-GUG-psbA

LSC · 122-393 · 272 bp

Back to Vauquelinia

Pi 0.0148

MarkerSeek score 57.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 272
variable_sites: 7
pi: 0.0148
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	340-389 bp	1.0	84.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	290-339 bp	1.0	84.2
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	354-403 bp	1.0	84.1
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	353-402 bp	1.0	84.1
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	355-404 bp	1.0	84.1