Hotspot

accD

LSC · 57852-59138 · 1287 bp

Pi 0.00925

MarkerSeek score 54.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1287
variable_sites: 24
pi: 0.00925
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAGAGGAAAGGGGGAAACT	TAAATGGACTTGCCCCATCG	50.0	50.0	57.9	57.9	2025-2129 bp	1.0	81.2
accD_p2	TCAGAGGAAAGGGGGAAACT	ACGATTGTGTATCTTGGCCC	50.0	50.0	57.9	58.0	2334-2438 bp	1.0	81.0
accD_p3	TCAGAGGAAAGGGGGAAACT	CGGAAGTACTAGGGCCACTA	50.0	55.0	57.9	57.9	2238-2342 bp	1.0	80.9
accD_p4	ATTGTTGTGTTGGGTCCACA	TAAATGGACTTGCCCCATCG	45.0	50.0	57.8	57.9	2244-2348 bp	1.0	80.8
accD_p5	ATTGTTGTGTTGGGTCCACA	ACGATTGTGTATCTTGGCCC	45.0	50.0	57.8	58.0	2553-2657 bp	1.0	80.6