Hotspot

ndhD-ccsA

SSC · 121441-121692 · 252 bp

Back to Vatica

Pi 0.041
MarkerSeek score 67.20
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
252
variable_sites
21
pi
0.041
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 TATCAGGTGAGAGGGTCGAC GCAGTCTGGGCTAATGAGTC 55.0 55.0 57.9 58.1 548 bp 1.0 82.5
ndhD-ccsA_p2 ACTATCAGGTGAGAGGGTCG GCAGTCTGGGCTAATGAGTC 55.0 55.0 57.9 58.1 550 bp 1.0 82.4
ndhD-ccsA_p3 TATCAGGTGAGAGGGTCGAC TTTGCTACTTCGGGCCTTTT 55.0 45.0 57.9 58.0 926 bp 1.0 81.6
ndhD-ccsA_p4 ACTATCAGGTGAGAGGGTCG TTTGCTACTTCGGGCCTTTT 55.0 45.0 57.9 58.0 928 bp 1.0 81.6
ndhD-ccsA_p5 CGGCTTGTTTATCTCGACGA TTTGCTACTTCGGGCCTTTT 50.0 45.0 58.1 58.0 1311 bp 1.0 80.8