Hotspot

trnN-GUU-rps15

SSC · 107040-113495 · 6456 bp

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Pi 0.00749
MarkerSeek score 60.30
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
SSC
length_bp
6456
variable_sites
108
pi
0.00749
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 ACCCCTTTCGTGTCATTGAC CGACCACAACAACCGAATTG 50.0 50.0 58.1 58.0 331-475 bp 1.0 87.8
trnN-GUU-rps15_p2 ACCCCCGTAACTTCTTCGTA CGACCACAACAACCGAATTG 50.0 50.0 58.1 58.0 442-586 bp 1.0 87.0
trnN-GUU-rps15_p3 TCGTAACCCCCGTAACTTCT CGACCACAACAACCGAATTG 50.0 50.0 58.1 58.0 447-591 bp 1.0 87.0
trnN-GUU-rps15_p4 GAGGTTTCCTTCGTAACCCC CGACCACAACAACCGAATTG 55.0 50.0 57.9 58.0 457-601 bp 1.0 86.7
trnN-GUU-rps15_p5 CAATTCCCGTTCTCAACCCA CGACCACAACAACCGAATTG 50.0 50.0 58.1 58.0 493-637 bp 1.0 86.5