Hotspot

trnP-UGG-psaJ

LSC · 66954-67372 · 419 bp

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Pi 0.0111
MarkerSeek score 58.50
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
419
variable_sites
11
pi
0.0111
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 AAAACAAACGCGCTACCAAG CACTTAGTACCGGTGCTACG 45.0 55.0 57.9 57.8 500-507 bp 1.0 80.8
trnP-UGG-psaJ_p2 CAAAACAAACGCGCTACCAA CACTTAGTACCGGTGCTACG 45.0 55.0 58.2 57.8 501-508 bp 1.0 80.3
trnP-UGG-psaJ_p3 TTGTACCCAAAACAAACGCG CACTTAGTACCGGTGCTACG 45.0 55.0 57.8 57.8 508-515 bp 1.0 80.1
trnP-UGG-psaJ_p4 AAAACAAACGCGCTACCAAG AATGTCAACGCATCGGGAAA 45.0 45.0 57.9 58.5 570-577 bp 1.0 78.1
trnP-UGG-psaJ_p5 AAAACAAACGCGCTACCAAG ATGTCAACGCATCGGGAAAA 45.0 45.0 57.9 58.5 569-576 bp 1.0 78.1