Hotspot

ndhC-trnV-UAC

LSC · 50718-51748 · 1031 bp

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Pi 0.00653
MarkerSeek score 58.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1031
variable_sites
15
pi
0.00653
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCCAAAACTCCAGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1162-1182 bp 1.0 77.8
ndhC-trnV-UAC_p2 TTTTCGAATCGGGGCCAAAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 58.7 57.8 1172-1192 bp 1.0 75.2
ndhC-trnV-UAC_p3 GGGGCCAAAACTCCAGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.4 57.5 1161-1181 bp 1.0 75.0
ndhC-trnV-UAC_p4 GGGGCCAAAACTCCAGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 58.4 58.7 1150-1170 bp 1.0 73.9
ndhC-trnV-UAC_p5 GGGGCCAAAACTCCAGAAAT AAGGTCTACGGTTCGAGTCC 50.0 55.0 58.4 58.8 1160-1180 bp 1.0 72.7