Hotspot

trnF-GAA-ndhJ

LSC · 48307-49022 · 716 bp

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Pi 0.00554
MarkerSeek score 58.40
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
716
variable_sites
9
pi
0.00554
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 CCTCGTGTCACCAGTTCAAA ATCCACGCCAGAAACGTATC 50.0 50.0 58.1 58.1 851-855 bp 1.0 81.1
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA CCACGCCAGAAACGTATCTT 50.0 50.0 58.1 58.3 849-853 bp 1.0 79.4
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA GAGAATCATCCACGCCAGAA 50.0 50.0 58.1 57.7 858-862 bp 1.0 79.1
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA CATCCACGCCAGAAACGTAT 50.0 50.0 58.1 58.3 852-856 bp 1.0 78.9
trnF-GAA-ndhJ_p5 CCTCGTGTCACCAGTTCAAA ATGCCCGAAAGTTGGATAGG 50.0 50.0 58.1 57.7 828-832 bp 1.0 78.9