Hotspot

rps4-trnT-UGU

LSC · 45784-46341 · 558 bp

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Pi 0.00287
MarkerSeek score 58.80
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
558
variable_sites
4
pi
0.00287
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 GATTGGTTTTTGCCGGCTTT CTCAGAGGTTAGAGCATCGC 45.0 55.0 58.1 57.9 717-719 bp 1.0 78.6
rps4-trnT-UGU_p2 GATTGGTTTTTGCCGGCTTT ATGGTCATCGGTTCGATTCC 45.0 50.0 58.1 57.8 685-687 bp 1.0 77.8
rps4-trnT-UGU_p3 GATTGGTTTTTGCCGGCTTT CAGAGGTTAGAGCATCGCAT 45.0 50.0 58.1 57.5 715-717 bp 1.0 75.5
rps4-trnT-UGU_p4 GATTGGTTTTTGCCGGCTTT TCAGAGGTTAGAGCATCGCA 45.0 50.0 58.1 58.5 716-718 bp 1.0 75.5
rps4-trnT-UGU_p5 TTGGTTTTTGCCGGCTTTAG CTCAGAGGTTAGAGCATCGC 45.0 55.0 57.5 57.9 715-717 bp 1.0 75.5