Hotspot

trnE-UUC-trnT-GGU

LSC · 29300-30257 · 958 bp

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Pi 0.0127
MarkerSeek score 72.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
958
variable_sites
23
pi
0.0127
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTAGTATCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.1 58.0 953-1096 bp 1.0 85.1
trnE-UUC-trnT-GGU_p2 CGTAGTATCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.1 58.1 932-1075 bp 1.0 84.6
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 902-1045 bp 1.0 84.6
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 900-1043 bp 1.0 84.6
trnE-UUC-trnT-GGU_p5 CGTAGTATCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.1 58.1 929-1072 bp 1.0 84.4