Hotspot

trnK-UUU-trnQ-UUG

LSC · 4258-6255 · 1998 bp

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Pi 0.00922
MarkerSeek score 64.60
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1998
variable_sites
38
pi
0.00922
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 CCGATGGTATGGACGAATCC TTTGGTCCCGGTGATCAAAG 55.0 50.0 57.9 58.1 2091-2162 bp 0.833333 77.3
trnK-UUU-trnQ-UUG_p2 CGATGGTATGGACGAATCCC TTTGGTCCCGGTGATCAAAG 55.0 50.0 57.9 58.1 2090-2161 bp 0.833333 77.3
trnK-UUU-trnQ-UUG_p3 GATGGTATGGACGAATCCCG TTTGGTCCCGGTGATCAAAG 55.0 50.0 57.9 58.1 2089-2160 bp 0.833333 77.3
trnK-UUU-trnQ-UUG_p4 CGATGGTATGGACGAATCCC CCGGTGATCAAAGGTTCGAA 55.0 50.0 57.9 58.2 2083-2154 bp 0.833333 76.5
trnK-UUU-trnQ-UUG_p5 GATGGTATGGACGAATCCCG CCGGTGATCAAAGGTTCGAA 55.0 50.0 57.9 58.2 2082-2153 bp 0.833333 76.5