Hotspot

psbA

LSC · 501-1562 · 1062 bp

Back to Vasconcellea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAAACGCATTGGTCTCCACT	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	58.0	58.4	1143 bp	1.0	76.8
psbA_p2	ACGCATTGGTCTCCACTAAG	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	57.6	58.4	1140 bp	1.0	73.9
psbA_p3	CGCATTGGTCTCCACTAAGA	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	57.3	58.4	1139 bp	1.0	71.6
psbA_p4	AAAACGCATTGGTCTCCACT	AGAATTTGTGTGCTTGGGAGT	45.0	42.9	58.0	58.1	1146 bp	1.0	71.4
psbA_p5	AAAACGCATTGGTCTCCACT	TTTGTGTGCTTGGGAGTCC	45.0	52.6	58.0	57.9	1142 bp	1.0	71.0