Hotspot

matK

LSC · 2183-3697 · 1515 bp

Back to Vasconcellea

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTGTTAGCTCTTCCGTCAGC	AACACCCTACCTTCAGTTCA	50.0	45.0	57.9	56.0	1616 bp	1.0	63.3
matK_p2	TTGTTAGCTCTTCCGTCAGC	ACACCCTACCTTCAGTTCAA	50.0	45.0	57.9	56.0	1615 bp	1.0	63.3
matK_p3	TTGTTAGCTCTTCCGTCAGC	ACCCTACCTTCAGTTCAAATCG	50.0	45.5	57.9	58.1	1613 bp	1.0	62.9
matK_p4	ATTGCCATCATTTCCACGGT	AACACCCTACCTTCAGTTCA	45.0	45.0	58.1	56.0	1585 bp	1.0	62.7
matK_p5	ATTGCCATCATTTCCACGGT	ACACCCTACCTTCAGTTCAA	45.0	45.0	58.1	56.0	1584 bp	1.0	62.7