Hotspot

ycf4-cemA

LSC · 63370-64331 · 962 bp

Back to Vasconcellea

Pi 0

MarkerSeek score 58.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	ACGAGAGAGAGATCCACCAG	45.0	55.0	58.1	57.9	1079-1080 bp	1.0	78.9
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	1071-1072 bp	1.0	78.7
ycf4-cemA_p3	AAAAAGCTGCTGAATTGGCC	ACGAGAGAGAGATCCACCAG	45.0	55.0	57.8	57.9	1093-1094 bp	1.0	78.3
ycf4-cemA_p4	AAAAAGCTGCTGAATTGGCC	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1085-1086 bp	1.0	78.1
ycf4-cemA_p5	AAAGCTGCTGAATTGGCCTA	ACGAGAGAGAGATCCACCAG	45.0	55.0	57.8	57.9	1091-1092 bp	1.0	77.9