Hotspot

ndhC-trnV-UAC

LSC · 53490-54145 · 656 bp

Back to Vasconcellea

Pi 0

MarkerSeek score 58.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGATTAGTCGATTCGAATTGG	GGTTCGAATCCGTATAGCCC	45.5	55.0	57.4	57.9	723-724 bp	1.0	58.5
ndhC-trnV-UAC_p2	CGGATTAGTCGATTCGAATTGG	CGGTTCGAATCCGTATAGCC	45.5	55.0	57.4	58.3	724-725 bp	1.0	56.6
ndhC-trnV-UAC_p3	CGGATTAGTCGATTCGAATTGG	ACTGCATGTTGGGTCTTTGA	45.5	45.0	57.4	57.6	801-802 bp	1.0	56.1
ndhC-trnV-UAC_p4	AGGAACTCCTATGAATGTGGA	GGTTCGAATCCGTATAGCCC	42.9	55.0	55.8	57.9	752-753 bp	1.0	53.5
ndhC-trnV-UAC_p5	CGGATTAGTCGATTCGAATTGG	CGAGAAGGTCTACGGTTCGA	45.5	55.0	57.4	58.9	736-737 bp	1.0	51.7