Hotspot

petN-psbM

LSC · 30141-30794 · 654 bp

Back to Vasconcellea

Pi 0.00153

MarkerSeek score 59.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GCACTCTTCATTCTCGTTCCT	50.0	47.6	58.2	58.0	809 bp	1.0	70.6
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACTGCACTCTTCATTCTCGT	50.0	45.0	58.2	56.9	812 bp	1.0	69.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	GCACTCTTCATTCTCGTTCCT	50.0	47.6	57.6	58.0	748 bp	1.0	69.0
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCACTCTTCATTCTCGTTCC	50.0	47.6	58.2	58.3	810 bp	1.0	68.4
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	ACTGCACTCTTCATTCTCGT	50.0	45.0	57.6	56.9	751 bp	1.0	68.4