Hotspot

rps16-trnQ-UUG

LSC · 6514-7149 · 636 bp

Back to Vasconcellea

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
636
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GGAGGTCAAAATCCACAGCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 747 bp 1.0 78.7
rps16-trnQ-UUG_p2 TGGAGGTCAAAATCCACAGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 748 bp 1.0 78.7
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 709 bp 1.0 78.3
rps16-trnQ-UUG_p4 CCTTCAATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 719 bp 1.0 77.5
rps16-trnQ-UUG_p5 GTGGTGGAGGTCAAAATCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 752 bp 1.0 77.1