Hotspot

trnK-UUU-rps16

LSC · 4498-5369 · 872 bp

Back to Vasconcellea

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
872
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG TATTTCAAAGAAGGCGGGGG 50.0 50.0 58.6 57.9 978 bp 1.0 74.3
trnK-UUU-rps16_p2 TAGTCGCACTTAAAAGCCGA TATTTCAAAGAAGGCGGGGG 45.0 50.0 57.3 57.9 979 bp 1.0 73.1
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA TATTTCAAAGAAGGCGGGGG 45.0 50.0 57.3 57.9 976 bp 1.0 73.1
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG TAAAAAGGGCGCTCAACCAA 50.0 45.0 58.6 58.3 1014 bp 1.0 73.0
trnK-UUU-rps16_p5 AGTCGCACTTAAAAGCCGAG TTAAAAAGGGCGCTCAACCA 50.0 45.0 58.6 58.3 1015 bp 1.0 73.0