Hotspot

ycf2

IRb · 90176-97432 · 7257 bp

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Pi 0.0302

MarkerSeek score 62.30

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 7257
variable_sites: 500
pi: 0.0302
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCCCATCTCCATCTCCTCAT	TTGTGGAGGAACAAGCCTTC	55.0	50.0	58.0	58.0	731 bp	0.2	47.5
ycf2_p2	CCCCATCTCCATCTCCTCAT	GACCGATGCACATCCAGATT	55.0	50.0	58.0	58.0	562 bp	0.2	47.3
ycf2_p3	CCCCATCTCCATCTCCTCAT	AGTGGAACAATCCCTGGAGA	55.0	50.0	58.0	58.0	617 bp	0.2	47.3
ycf2_p4	GTCGCGGACAGTGATTGTAT	TTGTGGAGGAACAAGCCTTC	50.0	50.0	58.1	58.0	1041 bp	0.2	47.2
ycf2_p5	GTCGCGGACAGTGATTGTAT	GACCGATGCACATCCAGATT	50.0	50.0	58.1	58.0	872 bp	0.2	47.1