Hotspot

trnN-GUU-ycf1

IRb · 109938-110473 · 536 bp

Back to Vanilla

Pi 0.0839

MarkerSeek score 79.90

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 536
variable_sites: 106
pi: 0.0839
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	TCCTAATGGTCAAAGCGAGC	GGTCGGCCCAATGCTAAATA	50.0	50.0	58.0	58.0	914 bp	0.2	47.6
trnN-GUU-ycf1_p2	TCCTAATGGTCAAAGCGAGC	AAAACCAGTTGTCGCTGCTA	50.0	45.0	58.0	58.0	846 bp	0.2	47.5
trnN-GUU-ycf1_p3	TCCTAATGGTCAAAGCGAGC	CCTAGTGAAAATGGGGGACG	50.0	55.0	58.0	58.0	1377 bp	0.2	47.5
trnN-GUU-ycf1_p4	TCCTAATGGTCAAAGCGAGC	CTAGTGAAAATGGGGGACGG	50.0	55.0	58.0	58.0	1376 bp	0.2	47.5
trnN-GUU-ycf1_p5	TCCTAATGGTCAAAGCGAGC	CCTTCTTCCATAACCCGAGC	50.0	55.0	58.0	58.0	812 bp	0.2	47.4