Hotspot

3'rps12-trnV-GAC

IRb · 100441-101407 · 967 bp

Back to Vanilla

Pi 0.0771

MarkerSeek score 78.40

Primer available yes

Label 3'rps12-trnV-GAC

Feature Metadata

label_name: 3'rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 967
variable_sites: 107
pi: 0.0771
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
3'rps12-trnV-GAC_p1	TCTACAACGCACTAGAACGC	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	1508-1875 bp	1.0	91.7
3'rps12-trnV-GAC_p2	TCTCACACCGGGTAAATCCT	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.1	58.0	1432-1799 bp	1.0	91.5
3'rps12-trnV-GAC_p3	CGAATACGGCTTTCCACAGA	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	1984-2351 bp	1.0	90.2
3'rps12-trnV-GAC_p4	TTGTCGGCGATCCTTTACTG	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	1853 bp	0.2	47.9
3'rps12-trnV-GAC_p5	TTGTCGGCGATCCTTTACTG	CGTCGCCTTGGTAAGCTATT	50.0	50.0	58.0	58.1	1932 bp	0.2	47.5