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trnL-CAA-rps7

IRb · 98049-99090 · 1042 bp

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Pi 0.0857

MarkerSeek score 77.50

Primer available yes

Label trnL-CAA-rps7

Feature Metadata

label_name: trnL-CAA-rps7
feature_type: igs
region: IRb
length_bp: 1042
variable_sites: 201
pi: 0.0857
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-rps7_p1	GTCTCGCGTGTCTACCATTT	ATGGCAGAGGCAAATAGAGC	50.0	50.0	58.0	58.0	1075-1113 bp	1.0	91.8
trnL-CAA-rps7_p2	CTCGCGTGTCTACCATTTCA	ATGGCAGAGGCAAATAGAGC	50.0	50.0	58.0	58.0	1073-1111 bp	1.0	91.8
trnL-CAA-rps7_p3	GTCTCGCGTGTCTACCATTT	GGCAGAGGCAAATAGAGCTT	50.0	50.0	58.0	58.0	1073-1111 bp	1.0	91.5
trnL-CAA-rps7_p4	CTCGCGTGTCTACCATTTCA	GGCAGAGGCAAATAGAGCTT	50.0	50.0	58.0	58.0	1071-1109 bp	1.0	91.5
trnL-CAA-rps7_p5	GCGTGTCTACCATTTCACCA	ATGGCAGAGGCAAATAGAGC	50.0	50.0	58.2	58.0	1070-1108 bp	1.0	90.3