Hotspot

rps8-rpl14

LSC · 82946-83757 · 812 bp

Back to Vanilla

Pi 0.0852

MarkerSeek score 81.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 133
pi: 0.0852
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTACAATCCCCATTCCGCC	GCCAAGAACTAAAACGCGAC	50.0	50.0	58.0	58.1	1193-1371 bp	1.0	91.5
rps8-rpl14_p2	ATTACAATCCCCATTCCGCC	CGATTGCTCGGGAATTGAGA	50.0	50.0	58.0	58.1	1088-1266 bp	1.0	91.5
rps8-rpl14_p3	TTCGACTCGGACTCTATCCC	GCCAAGAACTAAAACGCGAC	55.0	50.0	58.0	58.1	1619-1795 bp	1.0	91.3
rps8-rpl14_p4	TTCGACTCGGACTCTATCCC	CGATTGCTCGGGAATTGAGA	55.0	50.0	58.0	58.1	1514-1690 bp	1.0	91.2
rps8-rpl14_p5	ATTACAATCCCCATTCCGCC	ATCAGATCGACGGAAACACG	50.0	50.0	58.0	58.1	1724-1902 bp	1.0	90.7