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accD-psaI

LSC · 59461-60084 · 624 bp

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Pi 0.0901

MarkerSeek score 82.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 624
variable_sites: 92
pi: 0.0901
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTACCTTCCTCCTCCTTTG	GAGGGAAAGTTCAAATCTATCA	55.0	36.4	56.9	53.5	624-673 bp	1.0	52.0
accD-psaI_p2	GGTACCTTCCTCCTCCTTTGA	GAGGGAAAGTTCAAATCTATCA	52.4	36.4	58.5	53.5	624-673 bp	1.0	52.0
accD-psaI_p3	GGTACCTTCCTCCTCCTTTG	GAGGGAAAGTTCAAATCTATCAT	55.0	34.8	56.9	54.1	624-673 bp	1.0	52.0
accD-psaI_p4	GGTACCTTCCTCCTCCTTTG	AGGGAAAGTTCAAATCTATCA	55.0	33.3	56.9	52.1	623-672 bp	1.0	52.0
accD-psaI_p5	GGTACCTTCCTCCTCCTTTG	AGGGAAAGTTCAAATCTATCAT	55.0	31.8	56.9	52.8	623-672 bp	1.0	52.0