Hotspot

trnK-UUU-rps16

LSC · 4479-5237 · 759 bp

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Pi 0.047
MarkerSeek score 86.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
759
variable_sites
80
pi
0.047
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TGTTCATGATATTTTAAGGAAGGCA 50.0 32.0 58.2 57.0 836-995 bp 1.0 50.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TGTTCATGATATTTTAAGGAAGGCA 50.0 32.0 58.2 57.0 835-994 bp 1.0 50.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CTGTTCATGATATTTTAAGGAAGGCA 50.0 34.6 58.2 58.0 837-996 bp 1.0 50.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CTGTTCATGATATTTTAAGGAAGGCA 50.0 34.6 58.2 58.0 836-995 bp 1.0 50.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT ACTGTTCATGATATTTTAAGGAAGGC 50.0 34.6 58.2 58.0 837-996 bp 1.0 50.9