Hotspot

rps16

LSC · 4968-6153 · 1186 bp

Back to Vandopsis

Pi 0.0144

MarkerSeek score 52.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1186
variable_sites: 17
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CATTGAGTTAGCAACCCGGA	AAGTGAAATCCTTTCCGGGT	50.0	45.0	57.9	57.0	1823-1850 bp	1.0	72.4
rps16_p2	ACCATTGAGTTAGCAACCCG	AAGTGAAATCCTTTCCGGGT	50.0	45.0	58.2	57.0	1825-1852 bp	1.0	71.8
rps16_p3	CATTGAGTTAGCAACCCGGA	TGTATCGGTTCAGCCAATGA	50.0	45.0	57.9	56.9	1765-1792 bp	1.0	71.6
rps16_p4	CATTGAGTTAGCAACCCGGA	GTATCGGTTCAGCCAATGACT	50.0	47.6	57.9	58.1	1764-1791 bp	1.0	71.4
rps16_p5	ACCATTGAGTTAGCAACCCG	TGTATCGGTTCAGCCAATGA	50.0	45.0	58.2	56.9	1767-1794 bp	1.0	71.0