Hotspot

ycf1-rpl32

SSC · 111751-112213 · 463 bp

Back to Vandopsis

Pi 0.0485

MarkerSeek score 71.70

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 463
variable_sites: 20
pi: 0.0485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	GCTTCCTTCGTAACTCCAGG	TTCGTTCTCAGTCGGAGTCT	55.0	50.0	58.0	58.1	1288-1342 bp	1.0	84.5
ycf1-rpl32_p2	GCTTCCTTCGTAACTCCAGG	CTTTTTGACTGTCCGGTGGA	55.0	50.0	58.0	58.0	944 bp	0.5	59.6
ycf1-rpl32_p3	AAAGCGAGCCCTTTCATTCA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1129 bp	0.5	59.0
ycf1-rpl32_p4	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1128 bp	0.5	59.0
ycf1-rpl32_p5	TCAAAGCGAGCCCTTTCATT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1131 bp	0.5	59.0