Hotspot

rpoA-rps11

LSC · 80788-80871 · 84 bp

Back to Vandopsis

Pi 0.0357

MarkerSeek score 71.20

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 84
variable_sites: 3
pi: 0.0357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACGCTTGCTGTCTACTCTTG	AAGAGATGCGGCATTACGAG	50.0	50.0	57.9	58.1	277-285 bp	1.0	79.1
rpoA-rps11_p2	ACGCTTGCTGTCTACTCTTG	GAAGAGATGCGGCATTACGA	50.0	50.0	57.9	58.1	278-286 bp	1.0	79.1
rpoA-rps11_p3	GGACAGAACGAAACGACCAT	AAGAGATGCGGCATTACGAG	50.0	50.0	58.2	58.1	304-312 bp	1.0	78.0
rpoA-rps11_p4	GGACAGAACGAAACGACCAT	GAAGAGATGCGGCATTACGA	50.0	50.0	58.2	58.1	305-313 bp	1.0	77.9
rpoA-rps11_p5	ACGCTTGCTGTCTACTCTTG	CGTACGTGATGTAACACCCA	50.0	50.0	57.9	57.6	226-234 bp	1.0	77.7