Hotspot

ndhC-trnV-UAC

LSC · 51172-51874 · 703 bp

Back to Vandopsis

Pi 0.0088
MarkerSeek score 66.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
703
variable_sites
6
pi
0.0088
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTGGACCTTCCCTAAAATCCC AGAAGGTCTACGGTTCGAGT 52.4 50.0 57.7 57.8 837-846 bp 1.0 70.6
ndhC-trnV-UAC_p2 GGACCTTCCCTAAAATCCCAG AGAAGGTCTACGGTTCGAGT 52.4 50.0 57.7 57.8 835-844 bp 1.0 70.6
ndhC-trnV-UAC_p3 TGGACCTTCCCTAAAATCCCA AGAAGGTCTACGGTTCGAGT 47.6 50.0 58.3 57.8 836-845 bp 1.0 70.2
ndhC-trnV-UAC_p4 GGACCTTCCCTAAAATCCCA AGAAGGTCTACGGTTCGAGT 50.0 50.0 56.5 57.8 835-844 bp 1.0 68.9
ndhC-trnV-UAC_p5 TGGACCTTCCCTAAAATCCC AGAAGGTCTACGGTTCGAGT 50.0 50.0 56.5 57.8 836-845 bp 1.0 68.9