Hotspot

rpoB-trnC-GCA

LSC · 27228-28889 · 1662 bp

Back to Vandopsis

Pi 0.00259

MarkerSeek score 64.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1662
variable_sites: 4
pi: 0.00259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	CCGGGGATAAAGGATTTGCA	50.0	50.0	58.2	57.9	1698-1817 bp	1.0	82.3
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	ACCGGGGATAAAGGATTTGC	50.0	50.0	58.2	57.9	1699-1818 bp	1.0	82.3
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TCGAACCGGGGATAAAGGAT	50.0	50.0	58.2	58.2	1703-1822 bp	1.0	81.2
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTG	TTCGAACCGGGGATAAAGGA	50.0	50.0	58.2	58.4	1704-1823 bp	1.0	79.4
rpoB-trnC-GCA_p5	AAATTCTTCCGTCAAGCCCT	CCGGGGATAAAGGATTTGCA	45.0	50.0	57.4	57.9	1699-1818 bp	1.0	79.0