Hotspot

ycf2

Genome · 1-5485 · 5485 bp

Back to Vandenboschia

Pi 0.000912

MarkerSeek score 53.00

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 5485
variable_sites: 5
pi: 0.000912
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	AATCCGGCGAATCTTTCACG	ACGCGATTTCGATCTGAACA	50.0	45.0	59.0	58.0	168 bp	1.0	72.5
ycf2_p2	AATCCGGCGAATCTTTCACG	TCAAATCCCTCAACGCGATT	50.0	45.0	59.0	57.9	180 bp	1.0	71.6
ycf2_p3	TCCTAATCCGGCGAATCTTTC	ACGCGATTTCGATCTGAACA	47.6	45.0	57.9	58.0	172 bp	1.0	71.5
ycf2_p4	CCTAATCCGGCGAATCTTTCA	ACGCGATTTCGATCTGAACA	47.6	45.0	58.2	58.0	171 bp	1.0	71.1
ycf2_p5	ACAATTGGTTCAGGAGATGCA	ACGCGATTTCGATCTGAACA	42.9	45.0	57.8	58.0	231 bp	1.0	71.0